MEOUT ENZYME STAIN DIGESTOR
Kleenco utilizes naturally-occurring micro-organisms in Meout Stain Digester. The specific strains found in our enzyme products are Bacillus amyloliquefaciens, Bacillus Licheniformis, Bacillus subtilis, Bacillus megaterium and Bacillus pumilus.
Organisms in the genus Bacillus are isolated from the soil and decaying plant and leaf matter. These specially-selected bacteria produce enzymes that break apart sugars, fats and proteins present in waste to create energy and materials for their own growth.
All of these organisms are classified as completely safe around humans and animals. They are recognized by the American Type Culture Collection (ATCC) as Biosafety level 1 bacteria. The US Centers for Disease Control publishes Biosafety guidelines. Biosafety level 1 is described in the BMBL Reference manual as follows:
“BIOSAFETY 1 is suitable for work involving well-characterized agents not known to cause disease in healthy adult humans, and minimal potential hazard to laboratory personnel and the environment” (Biosafety in Microbiological and Biomedical Laboratories, U.S. Department of Health and Human Services, 3rd Edition, March 1993).
The safety of these organisms is further highlighted by the US Department of Health and Human Services, Food and Drug Administration in a final ruling which stated, in part, “The Food and Drug Administration (FDA) is affirming that carbohydrase enzyme preparations derived from either Bacillus subtilis or B. amyloliquefaciens are generally recognized as safe (GRAS) for use as direct food ingredients.”
In explaining this decision, they further state (part IV Section B. Corroborating Evidence of Safety), “Enzymes, including carbohydrase and protease enzymes in the enzyme preparations that are subject of this document, are naturally occurring proteins that are ubiquitous in living organisms,” and “Enzymes derived from microorganisms have been used as components of foods that have been safely consumed as part of the diet throughout human history” (Federal Register: April 23, 1999, Volume 64, Number 78, Docket No. 84G-0257).
The above-mentioned micro-organisms are aerobic spore-forming bacteria. This means they have an amazing ability to stop the normal, vegetative growth cycle when conditions become unfavorable (for example, the food runs out). The structure of the spore is shown in the illustration to the right. These spores are extremely resistant to just about anything you could mention. Freeze them, put them in a vacuum, expose them to high levels of UV or ionizing radiation, treat them with bleach, phenol, formaldehyde, boil them for hours, and they’ll still survive.
Such stability allows this product to be shipped long distances in any weather condition and stored for extended periods of time without special handling. The excellent stability of Meout Enzyme Stain Digester insures that the product is viable and effective until the last drop is used -- preventing waste and disappointing stain-removal results.
Rapid Germination and Outgrowth
Although shipped in a dormant state Meout Bacterial Enzymes retain an alert sensory mechanism that allows them to monitor their environment and respond to favorable conditions by germinating within minutes of exposure to specific germinants. This germination response consists of a complex series of biochemical events that result in the breaking of the dormant state and resumption of vegetative growth. The efficacy of this rapid germination is demonstrated just one minute from exposure to just 1 mM alanine (an amino acid).
Faster germination and outgrowth produces superior stain-removal results as the micro-organisms instantly release enzymes that catalyse the fundamental biochemical reaction that breaks apart organic stains and soils.
Synergistic Enzymatic Activity
Meout Stain Digester is formulated with micro-organisms that produce a synergistic combination of the following enzymes, protease, amylase, lipase, esterase, cellulase, and xylanase. Enzymes work like a key on a lock to break down large protein molecules into basic amino acids that are then easily removed from stained substrates.
Technically, all enzymes are organic catalysts that speed up reactions by lowering energies of activation. Most enzymes are stereo-selective, acting on a single substrate.
Because of the stereo selectivity of enzymes, it is important that stain-removal products be formulated with a diverse population of non-pathogenic bacteria capable of producing all enzymes necessary to catabolize all potential staining material.
The Meout enzyme combination is effective in breaking down protein, starch, carbohydrates, fats, grease and plant material. These macromolecules are the building blocks of food, soils, feces, vomit and other tenacious stains and soils. The specific activity of each enzyme is as follows:
Bacterial enzymes are bio-active; this means no outside energy source is needed for stain removal. The energy needed is produced when the enzymes break apart the substrate molecules, releasing the stored energy. No scrubbing or external heat is needed for this product to work.
Excessive rubbing or scrubbing damages fibers or protective finishes removes color and may spread the stain. Hot water or external heat sources may actually set stains. Being completely natural, Enzyme Stain Digester is much safer to use because no harsh, poisonous or flammable materials are encountered.
The efficacy or rate at which organic stains are removed from fabric or carpeting by microorganisms depends on numerous factors including nutrients, temperature, pH, doubling time and concentration of bacteria in the initial dose.
Meout Enzyme Stain Digester is highly efficient, with superior stain- removing performance. Each gallon of solution contains 200 billion bacteria. The doubling time for Bacillus subtilis is 30 minutes.
This means that once the bacteria have gone from a vegetative to an active state, each cell can grow from a single entity to a colony of over 1,000,000 in just 8 to 12 hours.
When properly applied, this product can remove a deeply-penetrated diarrhea feces stain from carpeting in as little as eight hours.
Technical Specifications for Meout Enzyme Stain Digester
Appearance Opaque, creamy-white liquid
Odor Fresh, pleasing fragrance
Optimum pH range 5.0 - 9.8
Bacteria Count 200 Billion/gallon
Bacteria Type Non-pathogenic bacillus spores
Bacterial Pathways Aerobic/facultative anaerobic
Specific Gravity 1.00
Freeze Thaw Stability Protect from freezing
Shelf Life Guaranteed one year
Environmentally Safe –
Because bacteria are naturally-occurring organisms, they pose no threat to the environment. Quite to the contrary, the environment benefits because the bacteria are being used to digest waste materials, breaking them down into end-products of totally inert carbon dioxide and water. Nature’s way of cleaning the environment.
Safe for use on any water-washable fabric. Effective on food, grease, oil, protein, and dirt-based stains, they can be used on any fabric and color and remain on fabric for up to one week.